yrvO

168

cysteine desulfurase, sulfur transfer to TrmU, involved in 2-thiouridine (s²U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules

Locus: BSU_27510

Molecular weight: 41.03 kDa

Isoelectric point: 5.46

Protein length: 379 aa

Gene length: 1140 bp

Function: tRNA modification

Product: cysteine desulfurase

Essential: yes

E.C.: 2.8.1.7

Synonyms: nifS, iscS, iscS1, YrvO

Genomic Context

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List of homologs in different organisms, belongs to COG1104 (Galperin et al., 2021)

Gene

Coordinates: 2,810,559  2,811,698

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules (together with TrmU) PubMed
• U34 in tRNA + L-cysteine + ATP --> (s²U34 in tRNA) + alanine + AMP + ppi PubMed

Protein family

• Class-V pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Cofactors

• PLP PubMed

Structure

• 1EG5 (PDB) (from Thermotoga maritima, 37% identity, 54% similarity) PubMed
• O34599 (AlphaFold)

Modification

• phosphorylated on Arg-385 PubMed

Paralogous protein(s)

• YcbU, NifZ, SufS, NifS

Expression and Regulation

Operons

• Genes: cymR-yrvO-trmU-yrrB-recD2

  Description: PubMed

• Genes: cymR-yrvO-trmU

  Description: PubMed

  Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A828 (yrvO::erm), available at the NBRP B. subtilis, Japan

References

Reviews

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Original Publications

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