azlD

168

amino acid exporter (with AzlC)

Locus: BSU_26700

Molecular weight: 11.83 kDa

Isoelectric point: 9.96

Protein length: 110 aa

Gene length: 333 bp

Function: export of amino acids

Product: component of amino acid exporter (with AzlC)

Essential: no

Synonyms: azlD, yrdI

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1687 (Galperin et al., 2021)

Gene

Coordinates: 2,728,647  2,728,979

The protein

Catalyzed reaction/ biological activity

• export of some branched-chain amino acids, histidine, and asparagine as well as 2,3-diaminopropionic acid (in complex with AzlD) PubMed

Protein family

• AzlD/HI_1737/HP1330 family (single member, according to UniProt)

Structure

• O07923 (AlphaFold)

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: azlB-azlC-azlD-brnQ-yrdK

  Description: PubMed

  Regulation

  • repressed by AzlB PubMed

  Regulatory mechanism

  • AzlB: repression, PubMed, in azlB regulon

Biological materials

Mutant

• GP3601 (ΔazlB-azlC-azlD::kan) , available in Jörg Stülke's lab PubMed
• GP3622 (ΔazlC-azlD::kan) , available in Jörg Stülke's lab PubMed
• GP3623 (ΔazlB-azlC-azlD::spec) , available in Jörg Stülke's lab PubMed
• GP4746 (trpC2 ΔazlD::neo), available in Jörg Stülke's lab
• GP4763 (trpC2 ΔazlD::lox72), available in Jörg Stülke's lab
• BKE26700 (azlD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGCTGCGTCATAGTCA,  downstream forward: _UP4_TAACCGATATCGGAAACGAT
• BKK26700 (azlD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGCTGCGTCATAGTCA,  downstream forward: _UP4_TAACCGATATCGGAAACGAT

References

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