smc

168

part of the condensin complex, chromosomal origin condensation and segregation SMC protein

Locus: BSU_15940

Molecular weight: 135.29 kDa

Isoelectric point: 5.27

Protein length: 1186 aa

Gene length: 3561 bp

Function: segregation of replication origins

Product: chromosome condensation and segregation SMC protein

Essential: yes

Synonyms: smc, ylqA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1196 (Galperin et al., 2021)

Gene

Coordinates: 1,666,560  1,670,120

Phenotypes of a mutant

• essential PubMed
• terminal phenotype of a CRISPRi knockdown mutant is moderate lysis PubMed
• ''smc'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth PubMed
• ''smc lethality on complex medim can be suppressed by inactivation of tmcAL and tsaC'' (constitutive stringent response) PubMed

The protein

Catalyzed reaction/ biological activity

• ATPase activity, cycles of ATP hydrolysis are used by the condensin complex to extrude DNA loops PubMed

Protein family

• SMC family (with SbcC, according to UniProt)

Domains

• SMC hinge domain (aa 519-637) (according to UniProt)

Structure

• 3ZGX (PDB) (complex between the head domain of Smc (aa 1 - 219 and 983 - 1186) and the N-terminal winged-helix domain of ScpA) PubMed
• P51834 (AlphaFold)

Effectors of protein activity

• ATP-induced DNA-binding results in a more open conformation of the Smc dimer, and thus of the condensin complex PubMed

Localization

• Nucleoid (heterogeneous) PubMed
• The condensin (Smc)₂-ScpA-ScpB complex is loaded by ParB to parS centromeric sites adjacent to the replication origin, travels then from the origin to the terminus at rates >50 kb per minute PubMed,PubMed,PubMed

Expression and Regulation

Operons

• Genes: rnc-smc-ftsY

  Description: PubMed

  Regulation

  • expressed during vegetative growth and during sporulation in the mother cell (SigK, GerE) PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B148 (smc::erm), available at the NBRP B. subtilis, Japan
• 1A822 ( smc::pMUTIN trpC2 Em), PubMed, available at the Bacillus Genetic Stock Center
• BEC15940 (smc::erm  trpC2), a CRISPRi knockdown mutant, PubMed, is available at the Bacillus Genetic Stock Center

References

Reviews

Loading

Original Publications

Loading