ispA

168

intracellular serine protease

Locus: BSU_13190

Molecular weight: 33.69 kDa

Isoelectric point: 4.52

Protein length: 319 aa

Gene length: 960 bp

Function: protein degradation

Product: intracellular serine protease

Essential: no

Synonyms: ispA, ispI

Genomic Context

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List of homologs in different organisms, belongs to COG1404 (Galperin et al., 2021)

Gene

Coordinates: 1,386,024  1,386,983

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth PubMed

The protein

Protein family

• peptidase S8 family (according to UniProt)

Domains

• Peptidase S8 domain (aa 23-307) (according to UniProt)

Structure

• 2WV7 (PDB) (from Bacillus clausii, 53% identity) PubMed
• P11018 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ispA

  Description: PubMed

  Regulation

  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in codY regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE13190 (yqiD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAAAAGCCCTCCTTTT,  downstream forward: _UP4_TAAGATTATTTTTCTTATAT
• BKK13190 (yqiD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAAAAGCCCTCCTTTT,  downstream forward: _UP4_TAAGATTATTTTTCTTATAT

References

Reviews

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Original Publications

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