acoC

168

acetoin dehydrogenase E2 component (dihydrolipoamide acetyltransferase)

Locus: BSU_08080

Molecular weight: 42.73 kDa

Isoelectric point: 6.52

Protein length: 398 aa

Gene length: 1197 bp

Function: acetoin utilization

Product: acetoin dehydrogenase E2 component (dihydrolipoamide acetyltransferase)

Essential: no

E.C.: 2.3.1.12

Synonyms: acoC, yfjI

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0508 (Galperin et al., 2021)

Gene

Coordinates: 881,049 → 882,245

The protein

Catalyzed reaction/ biological activity

• (R)-N⁶-dihydrolipoyl-L-lysyl-[protein] + acetyl-CoA --> (R)-N⁶-(S⁸-acetyldihydrolipoyl)-L-lysyl-[protein] + CoA (according to UniProt)

Protein family

• 2-oxoacid dehydrogenase family (according to UniProt)

Domains

• Lipoyl-binding domain (aa 2-77) (according to UniProt)
• Peripheral subunit-binding domain (PSBD) (aa 118-155) (according to UniProt)

Cofactors

• lipoic acid

Structure

• 3DUF (PDB) (PDH from Geobacillus stearothermophilus, 33% identity) PubMed
• O31550 (AlphaFold)

Paralogous protein(s)

• OdhB, BkdB, PdhC

Localization

• Membrane-proximal (Spotty) PubMed

Expression and Regulation

Operons

• Genes: acoA-acoB-acoC-acoL

  Description: PubMed

  Regulation

  • induced by acetoin (AcoR) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • AcoR: activation, (interaction with SigL-containing RNA polymerase) [ PubMed|11274109], in acoR regulon
  • Fnr: indirect positive regulation, in fnr regulon

  Sigma factors

  • SigL: sigma factor, PubMed, in sigL regulon

  Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Additional information

• the mRNA is very stable (> 15 min) PubMed

Biological materials

Mutant

• MGNA-C282 (acoC::erm), available at the NBRP B. subtilis, Japan
• BKE08080 (ΔacoC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTACCGCCATTTGTGTCC,  downstream forward: _UP4_TAGGAAAAGCAGGTGAAAAC
• BKK08080 (ΔacoC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTACCGCCATTTGTGTCC,  downstream forward: _UP4_TAGGAAAAGCAGGTGAAAAC

Labs working on this gene/protein

• Michel Debarbouille, Pasteur Institute, Paris, France Homepage

References

Reviews

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Original Publications

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