nadE

168

NH3-dependent NAD+ synthetase

Locus: BSU_03130

Molecular weight: 30.24 kDa

Isoelectric point: 4.89

Protein length: 272 aa

Gene length: 819 bp

Function: NAD biosynthesis

Product: NH3-dependent NAD+ synthetase

Essential: yes

E.C.: 6.3.1.5

Synonyms: nadE, outB, tscBGH, gsp-81

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0171 (Galperin et al., 2021)

Gene

Coordinates: 338,288  339,106

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + deamido-NAD⁺ + NH₄⁺ --> AMP + diphosphate + H⁺ + NAD⁺ (according to UniProt)

Protein family

• NAD synthetase family (single member, according to UniProt)

Structure

• 1EE1 (PDB) PubMed
• P08164 (AlphaFold)

Expression and Regulation

Operons

• Genes: nadE

  Description: PubMed

  Regulation

  • induced by antibiotics interacting with the cytoplasmic membrane PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon
  • SigA: sigma factor, in sigA regulon

References

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