yaaA

168

ribosome assembly factor, participates in the assembly of the 50S subunit of the ribosome

Locus: BSU_00030

Molecular weight: 7.79 kDa

Isoelectric point: 4.33

Protein length: 71 aa

Gene length: 216 bp

Function: assembly of the 50S subunit of the ribosome

Product: ribosome assembly factor

Synonyms: yaaA, , rlbA

Genomic Context

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List of homologs in different organisms, belongs to COG2501 (Galperin et al., 2021)

Gene

Coordinates: 3,206 → 3,421

Phenotypes of a mutant

• enhanced expression suppresses the defects of a temperature-sensitive ''rplB'' mutant PubMed
• the mutant cells are thicker and longer than wild type cells PubMed

The protein

Protein family

• S4 RNA-binding domain superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)

Domains

• S4 RNA-binding domain (aa 12-69) (according to UniProt)

Structure

• P05650 (AlphaFold)

Expression and Regulation

Operons

• Genes: yaaA-recF-remB-gyrB-gyrA

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B879 (yaaA::erm), available at the NBRP B. subtilis, Japan
• BKE00030 (ΔyaaA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATATCGACCTCTTTCA,  downstream forward: _UP4_GTCAATTAAAGCGGGTGACA
• BKK00030 (ΔyaaA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATATCGACCTCTTTCA,  downstream forward: _UP4_GTCAATTAAAGCGGGTGACA

References

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