yaaA

168

ribosome assembly factor, participates in the assembly of the 50S subunit of the ribosome

locus: BSU_00030

Molecular weight: 7.79 kDa

pI: 4.33

Protein length: 71 aa

Gene length: 216 bp

function: assembly of the 50S subunit of the ribosome

product: ribosome assembly factor

synonyms: yaaA, , rlbA

List of homologs in different organisms, belongs to COG2501 (Galperin et al., 2021)

Gene

Coordinates: 3,206 → 3,421

Phenotypes of a mutant

• enhanced expression suppresses the defects of a temperature-sensitive ''[gene|9445AC486A2B0A025030B275C789D227D5694145|rplB]'' mutant [Pubmed|24637032]
• the mutant cells are thicker and longer than wild type cells [pubmed|34846166]

The protein

Protein family

• [wiki|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)

[wiki|Domains]

• [wiki|S4 RNA-binding domain] (aa 12-69) (according to UniProt)

Structure

• [AF|P05650]

Expression and Regulation

Operons

• genes: [gene|FB1C21A94697E5B9D29C0F3B9C9F36E57BCBD237|yaaA]-[gene|EBE03AEC7EC594A3115A7A72194BDFF300AA0BFA|recF]-[gene|CBC76FB453569550F14FE5015C05D45985C04979|remB]-[gene|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|gyrB]-[gene|B207C7328177924B16F1C606F667BEADF12CA069|gyrA]

  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2987848], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-B879 (yaaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1878 NBRP B. subtilis, Japan]
• BKE00030 (Δ[gene|FB1C21A94697E5B9D29C0F3B9C9F36E57BCBD237|yaaA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00030 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATATCGACCTCTTTCA,  downstream forward: _UP4_GTCAATTAAAGCGGGTGACA
• BKK00030 (Δ[gene|FB1C21A94697E5B9D29C0F3B9C9F36E57BCBD237|yaaA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00030 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATATCGACCTCTTTCA,  downstream forward: _UP4_GTCAATTAAAGCGGGTGACA

References

• 2987848,24637032,34846166