ywbI

168

transcriptional regulator (LysR family), activator of the ywbH-ywbG operon

Locus: BSU_38310

Molecular weight: 34.52 kDa

Isoelectric point: 5.93

Protein length: 301 aa

Gene length: 906 bp

Function: control of expression of the ywbH-ywbG operon

Product: transcriptional regulator (LysR family)

Essential: no

Synonyms: ywbI, ipa-24d

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0583 (Galperin et al., 2021)

Gene

Coordinates: 3,932,198 → 3,933,103

The protein

Protein family

• LysR family (according to UniProt)

Domains

• HTH lysR-type domain (aa 1-58) (according to UniProt)

Structure

• 3FZV (PDB) (from Pseudomonas aeruginosa, 28% identity)
• P39592 (AlphaFold)

Effectors of protein activity

• acetate activates YwbI to bind DNA PubMed

Expression and Regulation

Operons

• Genes: ywbI-thiM-thiE

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B675 (ywbI::erm), available at the NBRP B. subtilis, Japan
• BKE38310 (ΔywbI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCGCTTCACCCTTTCTA,  downstream forward: _UP4_AAAGATAGTAAAGGATGATG
• BKK38310 (ΔywbI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCGCTTCACCCTTTCTA,  downstream forward: _UP4_AAAGATAGTAAAGGATGATG

References

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