ywtE

168

dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate (minor activity)

Locus: BSU_35850

Molecular weight: 31.44 kDa

Isoelectric point: 4.76

Protein length: 286 aa

Gene length: 861 bp

Function: dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate (minor activity)

Product: unknown

Essential: no

E.C.: 3.1.3.-

Synonyms: ywtE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0561 (Galperin et al., 2021)

Gene

Coordinates: 3,695,363  3,696,223

The protein

Catalyzed reaction/ biological activity

• dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate PubMed
• 5-amino-6-(5-phospho-D-ribitylamino)uracil + H₂O --> 5-amino-6-(D-ribitylamino)uracil + phosphate (according to UniProt)

Protein family

• HAD superfamily (according to UniProt)  PubMed
• Cof family (according to UniProt)

Structure

• 1NRW (PDB) (PhoC, 39% identity)
• P96741 (AlphaFold)

Expression and Regulation

Operons

• Genes: ywtE

  Description: PubMed

• Genes: tagT-ywtE

  Description: PubMed

  Regulation

  • expressed at high salt concentrations (SigM) PubMed

  Sigma factors

  • SigM: sigma factor, PubMed, in sigM regulon

Biological materials

Mutant

• MGNA-A546 (ywtE::erm), available at the NBRP B. subtilis, Japan
• BKE35850 (ywtE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGTAGGGGAACCTCCT,  downstream forward: _UP4_TAAAGAAAAGACTCCAGAGA
• BKK35850 (ywtE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGTAGGGGAACCTCCT,  downstream forward: _UP4_TAAAGAAAAGACTCCAGAGA

References

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