pncB

168

nicotinamide phosphoribosyltransferase, nicotinate phosphoribosyltransferase

Locus: BSU_31750

Molecular weight: 56.01 kDa

Isoelectric point: 5.09

Protein length: 490 aa

Gene length: 1473 bp

Function: NAD salvage and biosynthesis

Product: nicotinamide phosphoribosyltransferase

Essential: yes

E.C.: 2.4.2.11

Synonyms: pncB, yueK

Genomic Context

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List of homologs in different organisms, belongs to COG1488 (Galperin et al., 2021)

Gene

Coordinates: 3,259,403  3,260,875

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• 5-phospho-α-D-ribose 1-diphosphate + ATP + H₂O + nicotinamide --> nicotinamide mononucleotide + ADP + diphosphate + phosphate paper
• 5-phospho-α-D-ribose 1-diphosphate + ATP + H₂O + nicotinate --> ADP + diphosphate + nicotinate β-D-ribonucleotide + phosphate (according to UniProt)

Protein family

• NAPRTase family (single member, according to UniProt)

Structure

• 2F7F (PDB) (from Enterococcus faecalis, 61% identity)
• O32090 (AlphaFold)

Expression and Regulation

Operons

• Genes: pncA-pncB

  Description: PubMed

• Genes: yueH-yueI-pncA-pncB

  Description: PubMed

Biological materials

Mutant

• MGNA-B562 (yueK::erm), available at the NBRP B. subtilis, Japan

References

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