ytkP

168

similar to cysteine synthase

Locus: BSU_29970

Molecular weight: 32.91 kDa

Isoelectric point: 4.93

Protein length: 311 aa

Gene length: 933 bp

Function: unknown

Product: unknown

Essential: no

E.C.: 2.5.1.47

Synonyms: ytkP

Genomic Context

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List of homologs in different organisms, belongs to COG0031 (Galperin et al., 2021)

Gene

Coordinates: 3,066,451 → 3,067,386

The protein

Catalyzed reaction/ biological activity

• O(3)-acetyl-L-serine + H₂S --> L-cysteine + acetate (according to UniProt)

Protein family

• Cysteine synthase/cystathionine beta-synthase family (with CysK and MccA, according to UniProt)

Cofactors

• PLP (according to UniProt)

Structure

• 2EGU (PDB) (from Geobacillus Kaustophilus 57% identity)
• O34476 (AlphaFold)

Paralogous protein(s)

• CysK, MccA

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: ytkP

  Description: PubMed

Biological materials

Mutant

• MGNA-B534 (ytkP::erm), available at the NBRP B. subtilis, Japan
• BKE29970 (ΔytkP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTCATTCCTTTCTGAA,  downstream forward: _UP4_TAAAAAAAGGACTGGCTTCA
• BKK29970 (ΔytkP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTCATTCCTTTCTGAA,  downstream forward: _UP4_TAAAAAAAGGACTGGCTTCA