mmgF

168

methylisocitrate lyase

Locus: BSU_24120

Molecular weight: 32.94 kDa

Isoelectric point: 5.28

Protein length: 301 aa

Gene length: 906 bp

Function: mother cell metabolism

Product: methylisocitrate lyase

Essential: no

E.C.: 4.1.3.30

Synonyms: mmgF, yqiQ, prpB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2513 (Galperin et al., 2021)

Gene

Coordinates: 2,507,298  2,508,203

The protein

Catalyzed reaction/ biological activity

• 2-methyl-isocitrate --> pyruvate + succinate PubMed
• 3-hydroxybutane-1,2,3-tricarboxylate --> pyruvate + succinate (according to UniProt)

Protein family

• isocitrate lyase/PEP mutase superfamily (single member, according to UniProt)

Structure

• 1MUM (PDB) (enzyme of E. coli), 3KZ2 (PDB) (the enzyme from B. anthracis)
• P54528 (AlphaFold)

Expression and Regulation

Operons

• Genes: mmgA-mmgB-mmgC-mmgD-mmgE-mmgF

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed
  • strongly expressed during oligotrophic growth PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-C374 (yqiQ::erm), available at the NBRP B. subtilis, Japan
• BKE24120 (mmgF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGACATGAAACAAAATCCCC,  downstream forward: _UP4_TAATGCAACAAAAAAAGCCG
• BKK24120 (mmgF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGACATGAAACAAAATCCCC,  downstream forward: _UP4_TAATGCAACAAAAAAAGCCG

References

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