ycsE

168

5'-nucleotidase, dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate

Locus: BSU_04040

Molecular weight: 27.92 kDa

Isoelectric point: 4.49

Protein length: 249 aa

Gene length: 750 bp

Function: dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate

Product: 5'-nucleotidase

Essential: no

Synonyms: ycsE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0561 (Galperin et al., 2021)

Gene

Coordinates: 456,068 → 456,817

The protein

Catalyzed reaction/ biological activity

• dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate PubMed
• 5-amino-6-(5-phospho-D-ribitylamino)uracil + H₂O --> 5-amino-6-(D-ribitylamino)uracil + phosphate (according to UniProt)

Protein family

• HAD superfamily (according to UniProt) PubMed
• Cof family (according to UniProt)

Structure

• 1NRW (PDB) (PhoC, 31% identity)
• P42962 (AlphaFold)

Expression and Regulation

Operons

• Genes: ycsE

  Description: PubMed

Biological materials

Mutant

• MGNA-C071 (ycsE::erm), available at the NBRP B. subtilis, Japan
• BKE04040 (ΔycsE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATATGTACCTCTCTTTA,  downstream forward: _UP4_TAAAAAAAGAGAGTCCTAAG
• BKK04040 (ΔycsE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATATGTACCTCTCTTTA,  downstream forward: _UP4_TAAAAAAAGAGAGTCCTAAG

References

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