motB

168

H⁺-coupled MotA-MotB flagellar stator

Locus: BSU_13680

Molecular weight: 29.33 kDa

Isoelectric point: 6.65

Protein length: 261 aa

Gene length: 786 bp

Function: Motility and chemotaxis

Product: flagellar stator subunit

Essential: no

Synonyms: motB

Genomic Context

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Categories containing this gene/protein

Information processing, Genetics, Genetic competence

Lifestyles, Exponential and early post-exponential lifestyles, Motility and chemotaxis, Flagellar proteins

Lifestyles, Exponential and early post-exponential lifestyles, Genetic competence

Lifestyles, Exponential and early post-exponential lifestyles, Swarming

Groups of genes, Membrane proteins

List of homologs in different organisms, belongs to COG1360 (Galperin et al., 2021)

This gene is a member of the following regulons

SigD regulon

Gene

Coordinates: 1,433,676 → 1,434,461

Phenotypes of a mutant

increased DegU-P formation, resulting in reduced comK expression and strongly reduced genetic competence PubMed
loss of swimming and Swarming motility PubMed
mucoid phenotype due to the DegU-P activated overexpression of the ''capB-capC-capA-capE'' operon and resulting overproduction of poly-gamma-glutamate PubMed

The protein

Protein family

MotB family (with MotS, according to UniProt)

Structure

6YSF (the MotA₅-MotB₂ complex from Clostridium sporogenes) PubMed
4B62 (PDB) (from Pseudomonas aeruginosa, corresponds to aa 110 ... 255, 33% identity)
P28612 (AlphaFold)

Modification

protonation of Asp-24 is essential for torque generation PubMed

Paralogous protein(s)

MotS

Localization

anchored to the cell wall, extending through the cell membrane PubMed

Expression and Regulation

Operons

Genes: motA-motB

Description: PubMed

Sigma factors

SigD: sigma factor, PubMed, in sigD regulon

Biological materials

Mutant

available in Nicola Stanley-Wall's lab PubMed
BKE13680 (ΔmotB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCTCATGCTTCTTCTTCTTT,  downstream forward: _UP4_TAGCAAAAAAGGAAGCCTTG
BKK13680 (ΔmotB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCTCATGCTTCTTCTTCTTT,  downstream forward: _UP4_TAGCAAAAAAGGAAGCCTTG

References

Reviews

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Original Publications

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