opuBA

opuBA
168

choline and arsenocholine ABC transporter (ATP-binding protein)

Locus
BSU_33730
Molecular weight
42.92 kDa
Isoelectric point
5.65
Protein length
Gene length
Function
compatible solute transport
Product
choline and arsenocholine ABC transporter (ATP-binding protein)
Essential
no
Synonyms
opuBA, proV

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1125 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,462,105  3,463,250
The protein
Catalyzed reaction/ biological activity
uptake of choline and arsenocholine PubMed
Protein family
ABC transporter superfamily (according to UniProt)
2 CBS domains (aa 256-314, aa 316-374) (according to UniProt)
ABC transporter domain (aa 2-236) (according to UniProt)
Structure
2IT1 (PDB) (from Pyrococcus horikoshii, 43% identity)
Paralogous protein(s)
associated to the membrane (via OpuBB-OpuBD) PubMed
Expression and Regulation
Operons
Description
Regulation
induced by choline (GbsR) PubMed
Regulatory mechanism
GbsR: repression, (transcriptional roadblock) PubMed, in gbsR regulon
OpcR: repression, (sterical interference with RNA polymerase access) PubMed, in opcR regulon
RemA: activation, PubMed, in remA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
An antisense RNA is predicted for'opuBD' PubMed
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opuBAopuBD

2025-05-09 01:33:28

ghost

92

f5d474616565c5e32550f1c34aef09f972669a8e

1F7D81939583251B3B9B9F3D0D915C77CF89D428

Biological materials
Mutant
BKE33730 (opuBA::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CAAACATCGCACCTCCAAAA,  downstream forward: _UP4_TAGGGAGGGGCGATAAGTCA
BKK33730 (opuBA::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CAAACATCGCACCTCCAAAA,  downstream forward: _UP4_TAGGGAGGGGCGATAAGTCA
Expression vectors
pGP2938: (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
pGP3013: (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
LacZ fusion
pGP3880 (kan, based on pAC7]), available in Jörg Stülke's lab
GP4303 (opuBA-lacZ::aphA3), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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3F502A4DCB1DAD7C3F968465B13C35213240515B

Page visits: 4347

Time of last update: 2025-05-20 12:01:03

Author of last update: Jstuelk