Difference between revisions of "PBP137"
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[[File:Map_pBP137.png|250px|thumb|right|'''pBP137: click to enlarge''']] | [[File:Map_pBP137.png|250px|thumb|right|'''pBP137: click to enlarge''']] | ||
| − | + | [[File:PBP137_MCS.png|250px|thumb|right|'''pBP137 cloning region: click to enlarge''']] | |
Vector for the construction of translational ''gfp'' fusions that can be integrated at the ''amyE'' site | Vector for the construction of translational ''gfp'' fusions that can be integrated at the ''amyE'' site | ||
Latest revision as of 16:15, 29 October 2013
Vector for the construction of translational gfp fusions that can be integrated at the amyE site
Does not replicate in B. subtilis, integrates into the amyE site
Constructed in Fabian Commichau's lab
- in E. coli: ampicillin resistance
- in B. subtilis: chloramphenicol resistance
sequencing primers:
- KG307: 5’-CACCTTCACCCTCTCCACTGACAG -3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
