spoIVA

168

ATPase, spore coat morphogenetic protein, anchors the spore coat to the spore surface via SpoVM

Locus: BSU_22800

Molecular weight: 55.01 kDa

Isoelectric point: 4.55

Protein length: 492 aa

Gene length: 1479 bp

Function: initiation of spore coat assembly

Product: ATPase, basement layer protein for spore coat assembly

Essential: no

Synonyms: spoIVA, spoVP

Genomic Context

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List of homologs in different organisms, belongs to COG5831 (Galperin et al., 2021)

Gene

Coordinates: 2,386,195  2,387,673

Phenotypes of a mutant

• the spore coat does not localize to the spore surface but self-assembles into aggregates in the mother cell cytoplasm PubMed

The protein

Catalyzed reaction/ biological activity

• uses ATP hydrolysis to drive self-assembly into static filaments PubMed
• ATP hydrolysis drives polymerization of a nucleotide-free filament PubMed
• ploymerization depends on a critical threshold concentration of SpoIVA that is only achieved once the protein is recruited to the surface of the developing spore PubMed
• ATP + H₂O --> ADP + H⁺ + phosphate (according to UniProt)

Domains

• contains a Walker A ATPase domain

Structure

• P35149 (AlphaFold)

Modification

• phosphorylated on Arg-454 PubMed

Localization

• innermost protein of the spore coat basement PubMed

Expression and Regulation

Operons

• Genes: spoIVA

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• BKE22800 (spoIVA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGTGATCCCCTCCCGGAC,  downstream forward: _UP4_TAATACCGGTAGACCTCTTT
• BKK22800 (spoIVA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGTGATCCCCTCCCGGAC,  downstream forward: _UP4_TAATACCGGTAGACCTCTTT

References

Reviews

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Original Publications

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