trpD

168

anthranilate phosphoribosyltransferase

Locus: BSU_22670

Molecular weight: 35.87 kDa

Isoelectric point: 4.9

Protein length: 338 aa

Gene length: 1017 bp

Function: biosynthesis of tryptophan

Product: anthranilate phosphoribosyltransferase

Essential: no

E.C.: 2.4.2.18

Synonyms: trpD

Genomic Context

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List of homologs in different organisms, belongs to COG0547 (Galperin et al., 2021)

Gene

Coordinates: 2,374,881  2,375,897

The protein

Catalyzed reaction/ biological activity

• diphosphate + N-(5-phospho-β-D-ribosyl)anthranilate --> 5-phospho-α-D-ribose 1-diphosphate + anthranilate (according to UniProt)

Protein family

• anthranilate phosphoribosyltransferase family (single member, according to UniProt)

Structure

• 4YI7 (PDB) (from Acinetobacter sp., 42% identity)
• P03947 (AlphaFold)

Expression and Regulation

Operons

• Genes: trpE-trpD-trpC-trpF-trpB-trpA

  Description: PubMed

  Regulation

  • not expressed if tryptophan is available (MtrB) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
  • the RNA switch RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • MtrB: transcription termination, PubMed, in mtrB regulon

• Genes: trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE

  Description: PubMed

  Regulation

  • not expressed if tryptophan is available (MtrB) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

  Regulatory mechanism

  • MtrB: transcription termination, PubMed, in mtrB regulon

Biological materials

Mutant

• BKE22670 (trpD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTCACCGGCAGTAAGGGTTT,  downstream forward: _UP4_CTAAAGCAGAAAGAGGAAGA
• BKK22670 (trpD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTCACCGGCAGTAAGGGTTT,  downstream forward: _UP4_CTAAAGCAGAAAGAGGAAGA

References

Reviews

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Original Publications

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