nprE

168

extracellular neutral protease B

Locus: BSU_14700

Molecular weight: 56.35 kDa

Isoelectric point: 7.48

Protein length: 521 aa

Gene length: 1566 bp

Function: degradation of proteins

Product: extracellular neutral protease B

Essential: no

Synonyms: nprE

Genomic Context

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List of homologs in different organisms, belongs to COG3227 (Galperin et al., 2021)

Gene

Coordinates: 1,540,036  1,541,601

The protein

Catalyzed reaction/ biological activity

• Similar, but not identical, to that of thermolysin (according to UniProt)

Protein family

• peptidase M4 family (with NprB, according to UniProt)

Structure

• 1NPC (PDB) (of Bacillus cereus)
• P68736 (AlphaFold)

Paralogous protein(s)

• NprB

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: nprE

  Description: PubMed

  Regulation

  • repressed during exponential growth (ScoC) PubMed

  Regulatory mechanism

  • ScoC: repression, PubMed, in scoC regulon
  • AbrB: repression, PubMed, in abrB regulon
  • CodY: repression, PubMed, in codY regulon

  Additional information

  • in a triple ''abrB codY scoC'' mutant, expression occurs during exponential growth PubMed

Biological materials

Mutant

• KO7 (nprE  aprE  epr  mpr  nprB  vpr  bpr), available as BGSC 1A1133
• BKE14700 (nprE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAATAAATCCCCCTTTTT,  downstream forward: _UP4_TAATATTAGGAAAAGCCTGA
• BKK14700 (nprE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAATAAATCCCCCTTTTT,  downstream forward: _UP4_TAATATTAGGAAAAGCCTGA

References

Reviews

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Original Publications

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