appA/2

168

oligopeptide ABC transporter, inactive pseudogene in strain 168

Locus: BSU_11382

Protein length: 388 aa

Gene length: 1167 bp

Function: unknown

Product: oligopeptide ABC transporter

Essential: no

Synonyms: appA\/2

Genomic Context

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Gene

Coordinates: 1,214,001  1,215,167

The protein

Protein family

• bacterial solute-binding protein 5 family (according to UniProt)

Structure

• 1XOC (PDB) (the intact protein) PubMed

Expression and Regulation

Operons

• Genes: appD-appF-appA/1-appA/2-appB-appC

  Description: PubMed

  Regulation

  • repressed by CodY PubMed

  Regulatory mechanism

  • TnrA: activation, PubMed, in tnrA regulon
  • CodY: repression, PubMed PubMed, in codY regulon
  • ScoC: repression, PubMed, in scoC regulon

Biological materials

Mutant

• GP2100 (D(''appD-appF-appA/2-appB-appC)::spc''), available in Jörg Stülke's lab
• BKE11382 (appA/2::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCCTTTTTTTCTACGGATTT,  downstream forward: _UP4_TAAATTTCCCTTAAAGGGGA
• BKK11382 (appA/2::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCCTTTTTTTCTACGGATTT,  downstream forward: _UP4_TAAATTTCCCTTAAAGGGGA

References

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