yaaR

168

unknown

Locus: BSU_00300

Molecular weight: 16.58 kDa

Isoelectric point: 9.72

Protein length: 146 aa

Gene length: 441 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yaaR

Genomic Context

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List of homologs in different organisms, belongs to COG1728 (Galperin et al., 2021)

Gene

Coordinates: 40,213  40,653

Phenotypes of a mutant

• defective in swarming motility (reduced rate of colony expansion) PubMed

The protein

Structure

• 2QUP (PDB) (from Bacillus halodurans, 40% identity)
• P37539 (AlphaFold)

Expression and Regulation

Operons

• Genes: xpaC-yaaN-yaaO-tmk-darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC

  Description: PubMed

• Genes: darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC

  Description: PubMed

  Regulation

  • expressed during growth and the transition phase, expression is erduced in stationary phase PubMed

• Genes: tmk-darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC

  Description: PubMed

Biological materials

Mutant

• MGNA-B898 (yaaR::erm), available at the NBRP B. subtilis, Japan
• GP1713 (''darA-yaaR::cat''), available in Jörg Stülke's lab
• BKE00300 (''yaaR::erm'', available in the BGSC and in Jörg Stülke's lab) PubMed
• BKE00300 (yaaR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGTTTCAGATCCTTAAA,  downstream forward: _UP4_CTTTACACATAGAGAGTGAT
• BKK00300 (yaaR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGTTTCAGATCCTTAAA,  downstream forward: _UP4_CTTTACACATAGAGAGTGAT

Expression vectors

• pGP2673: expression of yaaR-Strep by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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