dnaC

168

replicative DNA helicase, part of the replisome

Locus: BSU_40440

Molecular weight: 50.44 kDa

Isoelectric point: 4.55

Protein length: 454 aa

Gene length: 1365 bp

Function: DNA replication

Product: replicative DNA helicase

Essential: yes

E.C.: 3.6.4.12

Synonyms: dnaC

Genomic Context

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List of homologs in different organisms, belongs to COG0305 (Galperin et al., 2021)

Gene

Coordinates: 4,157,471  4,158,835

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• stimulates DnaG primase activity PubMed
• ATP + H₂O --> ADP + H⁺ + phosphate (according to UniProt)

Protein family

• helicase family (according to UniProt)

Domains

• SF4 helicase domain (aa 179-445) (according to UniProt)

Structure

• 2R6A (PDB) (Geobacillus stearothermophilus),  2R6C (PDB),  2R6D (PDB),  2R6E (PDB) PubMed
• 4M4W (PDB) (the DnaC-DnaI-DnaG complex) PubMed
• P37469 (AlphaFold)

Localization

• Cytoplasm (Homogeneous) PubMed

Expression and Regulation

Operons

• Genes: dnaC

  Description: PubMed

Biological materials

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

References

Reviews

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Original Publications

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