ytdA

168

similar to UTP-glucose-1-phosphate uridylyltransferase

Locus: BSU_30850

Molecular weight: 30.03 kDa

Isoelectric point: 5.91

Protein length: 272 aa

Gene length: 819 bp

Function: unknown

Product: unknown

Essential: no

E.C.: 2.7.7.9

Synonyms: ytdA, yzwA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1210 (Galperin et al., 2021)

Gene

Coordinates: 3,154,735 → 3,155,553

The protein

Catalyzed reaction/ biological activity

• α-D-glucose 1-phosphate + H⁺ + UTP --> diphosphate + UDP-α-D-glucose (according to UniProt)

Protein family

• UDPGP type 2 family (with GtaB and YngB, according to UniProt)

Structure

• 7B1R (PDB) (YngB, 44% identity) PubMed
• 3JUJ (PDB) (UDP-glucose pyrophosphorylase from Helicobacter pylori, 43% identity) PubMed
• P42407 (AlphaFold)

Paralogous protein(s)

• YngB, GtaB

Localization

• outer spore coat PubMed

Expression and Regulation

Operons

• Genes: ytdA

  Description: PubMed

  Regulation

  • expressed late during sporulation in the mother cell (SigK) PubMed

  Sigma factors

  • SigK: sigma factor, in sigK regulon

Biological materials

Mutant

• MGNA-A281 (ytdA::erm), available at the NBRP B. subtilis, Japan
• BKE30850 (ΔytdA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTATTTTCCCCTCAGC,  downstream forward: _UP4_AAGAACCCCCAATAATTGGG
• BKK30850 (ΔytdA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTATTTTCCCCTCAGC,  downstream forward: _UP4_AAGAACCCCCAATAATTGGG

References

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