amyX

168

pullulanase (debranching enzyme)

Locus: BSU_29930

Molecular weight: 80.92 kDa

Isoelectric point: 5.8

Protein length: 718 aa

Gene length: 2157 bp

Function: starch degradation

Product: pullulanase

Essential: no

Synonyms: amyX

Genomic Context

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List of homologs in different organisms, belongs to COG1523 (Galperin et al., 2021)

Gene

Coordinates: 3,061,651  3,063,807

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen (according to UniProt)

Protein family

• glycosyl hydrolase 13 family (according to UniProt)

Structure

• 2E8Z (PDB) (complex with alpha cyclodextrin),  2E9B (PDB) (complex with maltose)
• C0SPA0 (AlphaFold)

Paralogous protein(s)

• GlgB

Expression and Regulation

Operons

• Genes: ytlP-ytlQ-ytlR-amyX

  Description: PubMed

  Sigma factors

  • SigD: sigma factor, PubMed, in sigD regulon

• Genes: amyX

  Description: PubMed

Biological materials

Mutant

• BKE29930 (amyX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGGAAGCTCTCTCCTCC,  downstream forward: _UP4_TGACGAAGTTTGTGCTATAG
• BKK29930 (amyX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGGAAGCTCTCTCCTCC,  downstream forward: _UP4_TGACGAAGTTTGTGCTATAG

References

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