ansB

168

L-aspartase

locus: BSU_23570

Molecular weight: 52.39 kDa

pI: 5.69

Protein length: 475 aa

Gene length: 1428 bp

function: [metabolite|aspartate] degradation

product: L-aspartase

essential: no

ec: 4.3.1.1

synonyms: ansB

List of homologs in different organisms, belongs to COG1027 (Galperin et al., 2021)

Gene

Coordinates: 2,454,347  2,455,774

The protein

Catalyzed reaction/ biological activity

• L-[metabolite|aspartate] --> [metabolite|fumarate] + NH₄⁺ (according to UniProt)

Protein family

• class-II fumarase/aspartase family (with [protein|A3A2EF3C95B833A11843553107D781EDEFF0BC41|citG], according to UniProt)

Structure

• [PDB|3R6Q] (from ''Bacillus'' sp. YM55-1, 72% identity, 92% similarity) [Pubmed|21661762]
• [AF|P26899]

Paralogous protein(s)

• [protein|A3A2EF3C95B833A11843553107D781EDEFF0BC41|citG]

Expression and Regulation

Operons

• genes: [gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]

  description: [Pubmed|1711029]

  regulation

  • expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
  • expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]

  regulatory mechanism

  • [protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]: repression, [Pubmed|11914346], in [regulon|protein:146947C974D62BCB282BA3F5B924B35695D75886|ansR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1711029], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

• genes: [gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]-[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]-[gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]

  description: [Pubmed|22383849]

  regulation

  • [protein|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]: expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]

  expression

  • expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
  • expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]

  regulatory mechanism

  • [protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]: transcription repression, [pubmed|11914346], in [regulon|protein:146947C974D62BCB282BA3F5B924B35695D75886|ansR regulon]

Biological materials

Mutant

• GP1153 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::''ermC'') available in [wiki|Jörg Stülke]'s lab
• GP4256 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::''cat'') available in [wiki|Jörg Stülke]'s lab
• BKE23570 ([gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAATCTTTAACCTTCTTT,  downstream forward: _UP4_TAAAATTTGTATAAAATACA
• BKK23570 ([gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAATCTTTAACCTTCTTT,  downstream forward: _UP4_TAAAATTTGTATAAAATACA

lacZ fusion

• pGP2274 (in [wiki|pAC5]) (GP2961), available in [wiki|Jörg Stülke]'s lab

References

• 8478318,1711029,1711029,11914346,21219666,22383849,38483038