Difference between revisions of "PGP574"

Latest revision as of 14:04, 3 June 2013

Vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the C-terminus of the expressed protein

host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Stülke lab

in E. coli: ampicillin resistance

based on pET3C

sequencing primer:
- T7: 5’-TAATACGACTCACTATAGGG -3’
- NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’

@@ Line 1: / Line 1: @@
-pGP574
+Vector for the expression of proteins in ''E. coli'', the [[plasmids|plasmid]] allows to fuse a Strep-tag to the C-terminus of the expressed protein
-vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the C-terminus of the expressed protein
+host: ''E. coli'' BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
-host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
 constructed in the [[Stülke]] lab
-in E. coli: ampicillin resistance
+in ''E. coli'': ampicillin resistance
 based on pET3C
-Schilling, O., Herzberg, C., Hertrich, T., Vörsmann, H., Jessen, D., Hübner, S., Titgemeyer, F. & Stülke, J. (2006) Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples. Nucl. Acids Res. 34: 6102-6115. [http://www.ncbi.nlm.nih.gov/sites/entrez/17074746 PubMed]
+similar [[plasmid]]: [[pGP172]]
+* sequencing primer:
+**'''T7:''' 5’-TAATACGACTCACTATAGGG -3’
+** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
+<pubmed> 17074746 </pubmed>
+==  Return to [[Plasmids]] ==