bsuRB

168

component of the BsuM DNA restriction system, similar to 5-methylcytosine-specific restriction enzyme

Locus: BSU_06100

Molecular weight: 39.88 kDa

Isoelectric point: 8.7

Protein length: 343 aa

Gene length: 1032 bp

Function: BsuM DNA restriction

Product: protection against foreign DNA

Essential: no

Synonyms: ydiS

Genomic Context

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List of homologs in different organisms, belongs to COG1401 (Galperin et al., 2021)

Gene

Coordinates: 660,596  661,627

The protein

Catalyzed reaction/ biological activity

• Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates (according to UniProt)

Structure

• 6HZ4 (PDB) (5-methylcytosine-specific restriction enzyme from E. coli, corresponds to aa 29 ... 223, 29% identity) PubMed
• O34885 (AlphaFold)

Expression and Regulation

Operons

• Genes: bsuRA-bsuRB-bsuRC

  Description: PubMed

Biological materials

Mutant

• MGNA-C208 (ydiS::erm), available at the NBRP B. subtilis, Japan
• DELTA0 (ydiS  trpC2), available at BGSC
• BKE06100 (bsuRB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAGTTCATTTCCCCTAT,  downstream forward: _UP4_TCGTCTTTTGTGAGTGATTA
• BKK06100 (bsuRB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAGTTCATTTCCCCTAT,  downstream forward: _UP4_TCGTCTTTTGTGAGTGATTA

References

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