nap

168

carboxylesterase NA

Locus: BSU_05440

Molecular weight: 33.80 kDa

Isoelectric point: 5.8

Protein length: 300 aa

Gene length: 903 bp

Function: lipid degradation

Product: carboxylesterase NA

Essential: no

E.C.: 3.1.1.1

Synonyms: nap, cesA

Genomic Context

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List of homologs in different organisms, belongs to COG0596 (Galperin et al., 2021)

Gene

Coordinates: 592,303 → 593,205

The protein

Catalyzed reaction/ biological activity

• carboxylic ester + H₂O --> alcohol + carboxylate (according to UniProt)

Protein family

• AB hydrolase superfamily (according to UniProt)

Domains

• AB hydrolase-1 domain (aa 58-286) (according to InterPro)

Structure

• 2R11 (PDB)
• P96688 (AlphaFold)

Paralogous protein(s)

• YbfK

Expression and Regulation

Operons

• Genes: nap

  Description: PubMed

Biological materials

Mutant

• BKE05440 (Δnap::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACAAATGCCCCTCCCAA,  downstream forward: _UP4_TAAGGGCTTATAAGATAGTG
• BKK05440 (Δnap::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACAAATGCCCCTCCCAA,  downstream forward: _UP4_TAAGGGCTTATAAGATAGTG

References

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