yabC

168

similar to 16S rRNA methyltransferase

Locus: BSU_00360

Molecular weight: 32.89 kDa

Isoelectric point: 5.52

Protein length: 292 aa

Gene length: 879 bp

Function: rRNA modification

Product: 16S rRNA methyltransferase

Essential: no

Synonyms: yabC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0313 (Galperin et al., 2021)

Gene

Coordinates: 43,921 → 44,799

The protein

Catalyzed reaction/ biological activity

• Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA (according to UniProt)
• cytidine1402 in 16S rRNA + S-adenosyl-L-methionine --> 2'-O-methylcytidine1402 in 16S rRNA + H⁺ + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• Methyltransferase superfamily (according to UniProt)

Structure

• 3KWP (PDB) (from Lactobacillus brevis, 51% identity)
• P37544 (AlphaFold)

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: xpaC-yaaN-yaaO-tmk-darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC

  Description: PubMed

• Genes: darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC

  Description: PubMed

  Regulation

  • expressed during growth and the transition phase, expression is erduced in stationary phase PubMed

• Genes: tmk-darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC

  Description: PubMed

Biological materials

Mutant

• MGNA-B903 (yabC::erm), available at the NBRP B. subtilis, Japan
• BKE00360 (ΔyabC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAGAATCCCCATGTCCGACT,  downstream forward: _UP4_TAAAAAAACGTTCTTGTGTC
• BKK00360 (ΔyabC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAGAATCCCCATGTCCGACT,  downstream forward: _UP4_TAAAAAAACGTTCTTGTGTC

References

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