cotF

168

spore coat protein

Locus: BSU_40530

Molecular weight: 18.58 kDa

Isoelectric point: 7.37

Protein length: 160 aa

Gene length: 483 bp

Function: resistance of the spore

Product: spore coat protein

Essential: no

E.C.: null

Synonyms: cotF

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5577 (Galperin et al., 2021)

Gene

Coordinates: 4,167,110 → 4,167,592

The protein

Protein family

• CotF family (according to UniProt)

Structure

• P23261 (AlphaFold)

Modification

• phosphorylated on Arg-155 PubMed

Localization

• inner spore coat  PubMed

Expression and Regulation

Operons

• Genes: cotF

  Description: PubMed

  Regulation

  • expressed late during sporulation in the mother cell (SigK, SpoIIID) PubMed

  Regulatory mechanism

  • SpoIIID: activation, PubMed, in spoIIID regulon

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon

Biological materials

Mutant

• 1S106 ( cotF::cat), PubMed, available at BGSC
• 1S107 ( cotF::cat), PubMed, available at BGSC
• BKE40530 (ΔcotF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGAAAATTCTCCTTTA,  downstream forward: _UP4_TAAAACAAGCAGCAAAGGAG
• BKK40530 (ΔcotF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGAAAATTCTCCTTTA,  downstream forward: _UP4_TAAAACAAGCAGCAAAGGAG

References

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