ywpE

168

similar to sortase, but lacking the membrane anchor

Locus: BSU_36340

Molecular weight: 11.00 kDa

Isoelectric point: 6.34

Protein length: 102 aa

Gene length: 309 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ywpE

Genomic Context

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List of homologs in different organisms, belongs to COG3764 (Galperin et al., 2021)

Gene

Coordinates: 3,741,732 → 3,742,040

The protein

Protein family

• bacterial sortase family (with YhcS, according to UniProt)

Structure

• 5HU4 (PDB) (sortase from Listeria monocytogenes, 49% identity) PubMed
• P94587 (AlphaFold)

Expression and Regulation

Operons

• Genes: ywpE

  Description: PubMed

  Regulation

  • strongly repressed during oligotrophic growth PubMed

Biological materials

Mutant

• MGNA-A588 (ywpE::erm), available at the NBRP B. subtilis, Japan
• BKE36340 (ΔywpE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTTGCCGCCCCTATCA,  downstream forward: _UP4_TAAATATGCAAGAGTGAAAG
• BKK36340 (ΔywpE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTTGCCGCCCCTATCA,  downstream forward: _UP4_TAAATATGCAAGAGTGAAAG

References

Reviews

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Research papers

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