nadA

168

quinolinate synthetase

Locus: BSU_27850

Molecular weight: 41.33 kDa

Isoelectric point: 5.72

Protein length: 368 aa

Gene length: 1107 bp

Function: NAD biosynthesis

Product: quinolinate synthetase

Essential: no

E.C.: 2.5.1.72

Synonyms: nadA

Genomic Context

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Categories containing this gene/protein

Metabolism, Additional metabolic pathways, Biosynthesis of cofactors, Biosynthesis of NAD(P)

List of homologs in different organisms, belongs to COG0379 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, NadR regulon

Gene

Coordinates: 2,845,955 → 2,847,061

The protein

Catalyzed reaction/ biological activity

ATP + L-threonyl-[protein] --> ADP + H⁺ + O-phospho-L-threonyl-[protein] (according to UniProt)

Protein family

quinolinate synthase A family (single member, according to UniProt)

Cofactors

Fe-S cluster PubMed

Structure

4HHE (PDB) (from Pyrococcus furiosus, 34% identity) PubMed
Q9KWZ1 (AlphaFold)

Expression and Regulation

Operons

Genes: nadB-nadC-nadA

Description: PubMed

Regulation

repressed in the presence of nicotinic acid (NadR) PubMed

Regulatory mechanism

NadR: repression, PubMed, in nadR regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Additional information

An ncRNA is predicted for 'nadB' PubMed

Biological materials

Mutant

BKE27850 (ΔnadA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCATTCGATTGTTTGA,  downstream forward: _UP4_TAGATCAGAAATATTTTCCC
BKK27850 (ΔnadA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCATTCGATTGTTTGA,  downstream forward: _UP4_TAGATCAGAAATATTTTCCC

Labs working on this gene/protein

Alessandra Albertini, University of Pavia, Italy homepage

References

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