cwlP

168

cell wall hydrolase of the SP-beta prophage region,

Locus: BSU_21350

Molecular weight: 252.01 kDa

Isoelectric point: 8.27

Protein length: 2285 aa

Gene length: 6858 bp

Function: cell wall turnover

Product: cell wall hydrolase

Essential: no

Synonyms: cwlP, yomI

Genomic Context

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List of homologs in different organisms, belongs to COG1196 (Galperin et al., 2021)

Gene

Coordinates: 2,249,154 → 2,256,011

The protein

Catalyzed reaction/ biological activity

• hydrolyzes the peptidoglycan and cell wall of B. subtilis PubMed

Protein family

• transglycosylase Slt family (together with CwlQ) (according to UniProt)

Domains

• contains soluble lytic transglycosylase (SLT) and peptidase M23 domains PubMed
• ten tetratrichopeptide repeats (according to UniProt)

Structure

• O31976 (AlphaFold)

Localization

• cell membrane (according to Swiss-Prot)

Biological materials

Mutant

• GP4480 (trpC2 ΔcwlP::neo), available in Jörg Stülke's lab
• BKE21350 (ΔcwlP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGTAAGAATCACTTCCTT,  downstream forward: _UP4_TAAGAGTCTGCAAAAGCAGA
• BKK21350 (ΔcwlP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGTAAGAATCACTTCCTT,  downstream forward: _UP4_TAAGAGTCTGCAAAAGCAGA

References

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