Difference between revisions of "Hfq"
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[miaA]]'', ''[[ymzC]]''  | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[miaA]]'', ''[[ymzC]]''  | ||
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| − | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17340 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17340 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17340   | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17340 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17340 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17340 DNA_with_flanks]  | 
|-  | |-  | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:ymaH_context.gif]]  | |colspan="2" | '''Genetic context''' <br/> [[Image:ymaH_context.gif]]  | ||
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>  |   <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>  | ||
|-  | |-  | ||
| − | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ymaH_1867373_1867594_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:hfq_expression.png|500px]]  | + | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ymaH_1867373_1867594_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:hfq_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17340]]  | 
|-  | |-  | ||
|}  | |}  | ||
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=== Database entries ===  | === Database entries ===  | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17340&redirect=T BSU17340]  | ||
* '''DBTBS entry:''' no entry  | * '''DBTBS entry:''' no entry  | ||
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=== Database entries ===  | === Database entries ===  | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17340&redirect=T BSU17340]  | ||
* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3HSB 3HSB] (complex with an RNA aptamer) {{PubMed|22053080}}  | * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3HSB 3HSB] (complex with an RNA aptamer) {{PubMed|22053080}}  | ||
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* '''Additional information:'''  | * '''Additional information:'''  | ||
| + | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 46 {{PubMed|24696501}}  | ||
=Biological materials =  | =Biological materials =  | ||
| Line 142: | Line 145: | ||
==Original publications==  | ==Original publications==  | ||
| − | <pubmed>12850135, 20445260 23457461 22965117,22053080</pubmed>  | + | <pubmed>12850135, 20445260 23457461 22965117,22053080 24932523 25150227 25915524</pubmed>  | 
[[Category:Protein-coding genes]]  | [[Category:Protein-coding genes]]  | ||
Latest revision as of 15:41, 29 April 2015
-  Description: RNA chaperone 
 
| Gene name | hfq | 
| Synonyms | ymaH | 
| Essential | no | 
| Product | RNA chaperone | 
| Function | unknown | 
| Gene expression levels in SubtiExpress: hfq | |
| MW, pI | 8 kDa, 8.698 | 
| Gene length, protein length | 219 bp, 73 aa | 
| Immediate neighbours | miaA, ymzC | 
| Sequences | Protein DNA DNA_with_flanks | 
 Genetic context  
  This image was kindly provided by SubtiList 
 | |
Expression at a glance   PubMed 
 | |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU17340
 
Phenotypes of a mutant
Database entries
- BsubCyc: BSU17340
 
- DBTBS entry: no entry
 
- SubtiList entry: [1]
 
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
 
- Protein family: hfq family (according to Swiss-Prot)
 
- Paralogous protein(s):
 
Extended information on the protein
- Kinetic information:
 
- Domains:
 
- Modification:
 
- Cofactor(s):
 
- Effectors of protein activity:
 
Database entries
- BsubCyc: BSU17340
 
- UniProt: O31796
 
- KEGG entry: [2]
 
- E.C. number:
 
Additional information
Expression and regulation
- Regulation:
 
- Regulatory mechanism:
 
-  Additional information:
- number of protein molecules per cell (complex medium with amino acids, without glucose): 46 PubMed
 
 
Biological materials
- Mutant: GP22 (cat), available in the Jörg Stülke's lab
 
- Expression vector:
 
- lacZ fusion: pGP460 (in pAC7), available in Jörg Stülke's lab
 
- GFP fusion:
 
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
 
-  FLAG-tag construct: 
- GP1067 (spc, based on pGP1331), available in Jörg Stülke's lab
 
 
- Antibody:
 
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications

