Difference between revisions of "PGP380"

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Vector for the overexpression of N-terminally Strep-tagged proteins in ''B. subtilis'', used for the SPINE technology
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[[File:PGP380.jpeg|250px|thumb|right|'''pGP380: click to enlarge''']]
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[[File:PGP380_cloning_region.jpeg|250px|thumb|right|'''pGP380 cloning region: click to enlarge''']]
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'''Application:'''
  
constructed in [[Stülke]] lab
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The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive overexpression of N-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and erythromycin in ''E. coli'' and ''B. subtilis'', respectively. pGP380 can be used for the [[SPINE]] method. [[pGP382]] is similar to the vector pGP380 which is derived from vectors [[pBQ200]] and [[pHT315]].
  
in ''E. coli'': ampicillin resistance
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Sequencing primers:
in B''. subtilis'': erythromycin resistance
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*'''M13_puc_for:''' 5‘-GTAAAACGACGGCCAGTG-3‘
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*'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘
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*'''FX125 (rev; primes -73bp of MCS):''' 5‘-GGCTCGTATGTTGTGTGG-3‘
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*'''JN54 (fwd; primes +148bp of MCS):''' 5‘-GTGAAAAATGAGCCGAAAGCAG-3‘
  
based on [[pBQ200]]
 
  
similar vector: [[pGP382]]
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<pubmed> 17994626 </pubmed>
  
Herzberg, C., Flórez Weidinger, L. A., Dörrbecker, B., Hübner, S., Stülke, J. & Commichau, F. M. (2007) SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics 7: 4032-4035. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17994626 PubMed]
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== Return to [[Plasmids]] ==

Latest revision as of 08:13, 29 July 2014

pGP380: click to enlarge
pGP380 cloning region: click to enlarge

Application:

The vector was constructed in the Stülke lab and it is suitable for constitutive overexpression of N-terminally Strep-tagged proteins in B. subtilis. The plasmid confers resistance to ampicillin and erythromycin in E. coli and B. subtilis, respectively. pGP380 can be used for the SPINE method. pGP382 is similar to the vector pGP380 which is derived from vectors pBQ200 and pHT315.

Sequencing primers:

  • M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
  • M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘
  • FX125 (rev; primes -73bp of MCS): 5‘-GGCTCGTATGTTGTGTGG-3‘
  • JN54 (fwd; primes +148bp of MCS): 5‘-GTGAAAAATGAGCCGAAAGCAG-3‘


Christina Herzberg, Lope Andrés Flórez Weidinger, Bastian Dörrbecker, Sebastian Hübner, Jörg Stülke, Fabian M Commichau
SPINE: a method for the rapid detection and analysis of protein-protein interactions in vivo.
Proteomics: 2007, 7(22);4032-5
[PubMed:17994626] [WorldCat.org] [DOI] (P p)


Return to Plasmids