nasB

168

nitrate reductase (electron transfer subunit)

Locus: BSU_03320

Molecular weight: 84.57 kDa

Isoelectric point: 5.48

Protein length: 771 aa

Gene length: 2316 bp

Function: utilization of nitrate

Product: nitrate reductase (electron transfer subunit)

Essential: no

Synonyms: nasB, nasBA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1251 (Galperin et al., 2021)

Gene

Coordinates: 360,442  362,757

The protein

Cofactors

• FAD  PubMed

Structure

• 3KLJ (PDB) (from Clostridium acetobutylicum, corresponds to aa 2 ... 371, 27% identity) PubMed
• P42433 (AlphaFold)

Paralogous protein(s)

• NasD

Expression and Regulation

Operons

• Genes: nasB-nasC-nasD-nasE-nasF

  Description: PubMed

  Regulation

  • ''nasD'': expressed under anaerobic conditions (ResD) PubMed

  Regulatory mechanism

  • ResD: activation, PubMed, in resD regulon
  • TnrA: activation, PubMed, in tnrA regulon
  • NsrR: repression, PubMed, in nsrR regulon
  • Fur: repression, PubMed, in fur regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• 1A971 ( nasB::erm), PubMed, available at BGSC
• BKE03320 (nasB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTATCAGACCTCCTTTG,  downstream forward: _UP4_TGAGGCATTTTGACTGAACG
• BKK03320 (nasB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTATCAGACCTCCTTTG,  downstream forward: _UP4_TGAGGCATTTTGACTGAACG

References

Reviews

Loading

Original Publications

Loading