glmM

168

phosphoglucosamine mutase, required for cell wall synthesis, inhibits CdaA activity

Locus: BSU_01770

Molecular weight: 48.27 kDa

Isoelectric point: 4.68

Protein length: 448 aa

Gene length: 1347 bp

Function: cell wall synthesis, control of c-di-AMP synthesis

Product: phosphoglucosamine mutase, required for cell wall synthesis

Essential: yes

E.C.: 5.4.2.10

Synonyms: glmM, ybbT

Genomic Context

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List of homologs in different organisms, belongs to COG1109 (Galperin et al., 2021)

Gene

Coordinates: 198,497 → 199,843

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• D-glucosamine 6-phosphate --> α-D-glucosamine 1-phosphate (according to UniProt)
• inhibition of CdaA activity (PubMed) under conditions of osmotic stress (demonstrated in L. monocytogenes ) PubMed

Protein family

• phosphohexose mutase family (with PgcA, PubMed)

Structure

• 7OML (PDB)
• 7OLH (PDB), 7OJS (PDB) (complex of the CdaA DAC domain and GlmM)
• 3PDK (PDB) (from B. anthracis) PubMed
• O34824 (AlphaFold)

Modification

• phosphorylation on Ser-100 PubMed, this is an autophosphorylation  PubMed

Expression and Regulation

Operons

• Genes: cdaA-cdaR-glmM-glmS

  Description: PubMed

  Regulatory mechanism

  • GlmS ribozyme: RNA switch, in GlmS ribozyme

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Expression vectors

• pGP400: (expression in B. subtilis in pBQ200), available in Jörg Stülke's lab
• pGP1401: (expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
• pGP1402: (cloning vector for glmM (GlmM S100A) in E. coli, in pBlueskript KS), available in Jörg Stülke's lab
• pGP1403: (expression of GlmM (S100A) in B. subtilis in pBQ200), available in Jörg Stülke's lab
• pGP1405: (expression, purification of GlmM (S100A) in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

• GP1380 lacA::cdaR-Strep aphA3 glmM-3xFLAG ermC (based on pGP1460 and pGP1087), available in Jörg Stülke's lab

LacZ fusion

• GP1340 (cat) based on pAC6, available in Jörg Stülke's lab PubMed

Antibody

• available in Jörg Stülke's lab

GFP fusion

• GP1382 glmM-gfp ermC (based on pGP1080), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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