ddcP

168

DNA damage checkpoint recovery protease, promotes DNA damage checkpoint recovery

Locus: BSU_15050

Molecular weight: 38.61 kDa

Isoelectric point: 5.85

Protein length: 341 aa

Gene length: 1026 bp

Function: degradation of YneA

Product: DNA damage checkpoint recovery protease

Essential: no

Synonyms: ddcP, ylbL

Genomic Context

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List of homologs in different organisms, belongs to COG3480 (Galperin et al., 2021)

Gene

Coordinates: 1,572,765  1,573,790

Phenotypes of a mutant

• deletion of CtpA and DdcP leads to accumulation of the checkpoint protein YneA PubMed
• sensitivity to DNA damage PubMed

The protein

Protein family

• Peptidase S16 family (with LonA and LonB, according to UniProt)

Domains

• TM helix (aa 9 - 29) PubMed
• PDZ domain (aa 100 - 186) PubMed
• Lon protease domain (aa 187 - 336) PubMed

Structure

• 2KJP (PDB) (PDZ domain)
• O34470 (AlphaFold)

Localization

• cell membrane, with extracellular protease domain PubMed

Expression and Regulation

Operons

• Genes: ylbK-ddcP

  Description: PubMed

• Genes: ddcP

  Description: PubMed

Biological materials

Mutant

• MGNA-B249 (ylbL::erm), available at the NBRP B. subtilis, Japan
• BKE15050 (ddcP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCTAAAATGTTTTTTACGTA,  downstream forward: _UP4_CTGAAAGCGAAAAGCACCTG
• BKK15050 (ddcP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCTAAAATGTTTTTTACGTA,  downstream forward: _UP4_CTGAAAGCGAAAAGCACCTG

References

Reviews

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Original Publications

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