nrgB

168

nitrogen-regulated PII protein

Locus: BSU_36520

Molecular weight: 12.68 kDa

Isoelectric point: 8.82

Protein length: 116 aa

Gene length: 351 bp

Function: regulation of ammonium uptake

Product: nitrogen-regulated PII protein

Essential: no

Synonyms: nrgB, glnK

Genomic Context

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Categories containing this gene/protein

Metabolism, Amino acid/ nitrogen metabolism, Biosynthesis/ acquisition of amino acids, Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation

Information processing, Regulation of gene expression, Transcription factors and their control, Control of transcription factor (other than two-component system)

Groups of genes, Membrane proteins

List of homologs in different organisms, belongs to COG0347 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, TnrA regulon

Gene

Coordinates: 3,758,016 → 3,758,366

The protein

Protein family

P(II) protein family (single member, according to UniProt)

Structure

4R25 (PDB) PubMed
4R25 PubMed
2NUU (the complex with NrgA, from E. coli) PubMed
Q07428 (AlphaFold)

Localization

cell membrane, via NrgA PubMed

Expression and Regulation

Operons

Genes: nrgA-nrgB

Description: PubMed

Regulation

induced by ammonium limitation

Regulatory mechanism

TnrA: activation, PubMed, in tnrA regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

GP4167 (ΔnrgA-nrgB::spec), available in  Jörg Stülke's lab
GP4168 (ΔnrgB::phleo), available in  Jörg Stülke's lab
GP259 (ΔnrgB::cat), available in Jörg Stülke's lab PubMed
GP255 (ΔnrgA-nrgB::cat), available in Jörg Stülke's lab PubMed
GP4092 (ΔnrgB::phleo), available in Jörg Stülke's lab
BKE36520 (ΔnrgB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGACCGCTCATAGCGTCAC,  downstream forward: _UP4_TAATATCGGTACGAGATTCG
BKK36520 (ΔnrgB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGACCGCTCATAGCGTCAC,  downstream forward: _UP4_TAATATCGGTACGAGATTCG

Antibody

available in Jörg Stülkes lab PubMed

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

References

Reviews

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Original Publications

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