yqjB

168

putative sporulation-specific L,D-transpeptidase

Locus: BSU_23940

Molecular weight: 19.55 kDa

Isoelectric point: 5.27

Protein length: 176 aa

Gene length: 531 bp

Function: unknown

Product: putative L,D-transpeptidase

Essential: no

Synonyms: yqjB

Genomic Context

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List of homologs in different organisms, belongs to COG1376 (Galperin et al., 2021)

Gene

Coordinates: 2,488,953  2,489,483

The protein

Protein family

• YkuD family (with YciB and Ldt, according to UniProt)

Structure

• 4A1I (PDB) (Ldt, 41% identity)
• P54539 (AlphaFold)

Paralogous protein(s)

• Ldt

Localization

• contains a signal peptide for secretion (according to UniProt)

Expression and Regulation

Operons

• Genes: yqjB-yqjC

  Description: PubMed

  Regulatory mechanism

  • SpoVT: repression, PubMed, in spoVT regulon

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

Biological materials

Mutant

• MGNA-C385 (yqjB::erm), available at the NBRP B. subtilis, Japan
• BKE23940 (yqjB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAGCTCCTCCTTTTTCA,  downstream forward: _UP4_TAAGGCGCCTGCTTTTTTAT
• BKK23940 (yqjB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAGCTCCTCCTTTTTCA,  downstream forward: _UP4_TAAGGCGCCTGCTTTTTTAT