murP

168

N-acetyl muramic acid permease of the phosphotransferase system, EIIBC of the PTS

Locus: BSU_01680

Molecular weight: 47.44 kDa

Isoelectric point: 8.5

Protein length: 455 aa

Gene length: 1368 bp

Function: N-acetyl muramic acid uptake and phosphorylation

Product: N-acetyl muramic acid PTS permease, EIIBC component

Essential: no

E.C.: 2.7.1.69

Synonyms: murP, ybbF

Genomic Context

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Categories containing this gene/protein

Cellular processes, Cell envelope and cell division, Cell wall degradation/ turnover, Utilization of cell wall components

Cellular processes, Transporters, Phosphotransferase system, Sugar specific PTS proteins

Metabolism, Carbon metabolism, Utilization of specific carbon sources, Utilization of amino sugars

Metabolism, Amino acid/ nitrogen metabolism, Utilization of nitrogen sources other than amino acids, Utilization of amino sugars

Groups of genes, Phosphoproteins, Phosphorylation on a Cys residue

List of homologs in different organisms, belongs to COG1264 (Galperin et al., 2021)

This gene is a member of the following regulons

MurR regulon

Gene

Coordinates: 189,790  191,157

The protein

Catalyzed reaction/ biological activity

transport and concomitant phosphorylation of N-acetyl muramic acid

Protein family

PTS permease, sucrose family PubMed

Domains

PTS EIIB domain type-1 (aa 8-90) (according to UniProt)
PTS EIIC domain type-1 (aa 116-455) (according to UniProt)

Structure

Q797S1 (AlphaFold)

Paralogous protein(s)

BglP, SacX, SacP, TreP

Expression and Regulation

Operons

Genes: murQ-murR-murP-amiE-nagZ-namZ

Description: PubMed

Regulation

expressed in late exponential and early stationary phase PubMed

Regulatory mechanism

MurR: repression, PubMed, in murR regulon

Biological materials

Mutant

MGNA-B950 (ybbF::erm), available at the NBRP B. subtilis, Japan
BKE01680 (murP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAAAACCCCTCCCCTTC,  downstream forward: _UP4_TAAATTGTTTCTGTTTTAGG
BKK01680 (murP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAAAACCCCTCCCCTTC,  downstream forward: _UP4_TAAATTGTTTCTGTTTTAGG

References

Reviews

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Original Publications

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