uppS

uppS
168

undecaprenyl pyrophosphate synthetase, provides the precursor for the carrier lipid, undecaprenyl phosphate (C55-P), which is required for cell wall synthesis

Locus
BSU_16530
Molecular weight
29.63 kDa
Isoelectric point
5.41
Protein length
Gene length
Function
cell wall biosynthesis
Product
undecaprenyl pyrophosphate synthetase
Essential
yes
E.C.
2.5.1.31
Synonyms
uppS, yluA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0020 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,721,214  1,721,996
Phenotypes of a mutant
essential PubMed
uppS is dispensable in wall-less L-forms PubMed
the uppS86 (C257T) mutation provides resistance to rare earth metals such as scandium and to bacitracin PubMed
inactivation suppresses the essentiality of mbl PubMed
The protein
Catalyzed reaction/ biological activity
catalyzes eight consecutive condensation reactions of isopentenyl pyrophosphate (IPP) with farnesyl pyrophosphate (FPP) to form undecaprenyl pyrophosphate
Protein family
UPP synthetase family (single member, according to UniProt)
Structure
1X09 (PDB) (the protein from E. coli, 49% identity, 77% similarity) PubMed
Expression and Regulation
Operons
Description
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uppSpolC

2025-06-17 12:26:17

Jstuelk

149

c6a20b58d42a343484ae9ff8f9312018bf0f82f2

3AF78B34597AE93B1AF63769A0A2561D91069003

Biological materials
Mutant
MGNA-B080 (yluA::erm), available at the NBRP B. subtilis, Japan
References
Englehart K, Dworkin JBacillus subtilis MurJ and Amj Lipid II flippases are not essential for growth.Journal of bacteriology. 2025 Apr 4; :e0007825. PMID: 40183557
Koo BM, Todor H, Sun J, van Gestel J, Hawkins JS, Hearne CC, Banta AB, Huang KC, Peters JM, Gross CAComprehensive double-mutant analysis of the Bacillus subtilis envelope using double-CRISPRi.bioRxiv : the preprint server for biology. 2024 Aug 16; . PMID: 39185233
O'Reilly FJ, Graziadei A, Forbrig C, Bremenkamp R, Charles K, Lenz S, Elfmann C, Fischer L, Stülke J, Rappsilber JProtein complexes in cells by AI-assisted structural proteomics.Molecular systems biology. 2023 Feb 23; :e11544. PMID: 36815589
Guo T, Herman JKMagnesium Modulates Bacillus subtilis Cell Division Frequency.Journal of bacteriology. 2022 Dec 14; :e0037522. PMID: 36515540
Workman SD, Day J, Farha MA, El Zahed SS, Bon C, Brown ED, Organ MG, Strynadka NCJStructural Insights into the Inhibition of Undecaprenyl Pyrophosphate Synthase from Gram-Positive Bacteria.Journal of medicinal chemistry. 2021 Sep 2; . PMID: 34473495
Wang Y, Desai J, Zhang Y, Malwal SR, Shin CJ, Feng X, Sun H, Liu G, Guo RT, Oldfield E Bacterial Cell Growth Inhibitors Targeting Undecaprenyl Diphosphate Synthase and Undecaprenyl Diphosphate Phosphatase. ChemMedChem. 2016 Oct 19; 11(20):2311-2319. doi:10.1002/cmdc.201600342. PMID:27578312
Kawai Y, Mercier R, Errington J Bacterial cell morphogenesis does not require a preexisting template structure. Current biology : CB. 2014 Apr 14; 24(8):863-7. doi:10.1016/j.cub.2014.02.053. pii:S0960-9822(14)00251-6. PMID:24704074
Hess BM, Xue J, Markillie LM, Taylor RC, Wiley HS, Ahring BK, Linggi B Coregulation of Terpenoid Pathway Genes and Prediction of Isoprene Production in Bacillus subtilis Using Transcriptomics. PloS one. 2013; 8(6):e66104. doi:10.1371/journal.pone.0066104. PMID:23840410
Lee YH, Helmann JD Reducing the Level of Undecaprenyl Pyrophosphate Synthase Has Complex Effects on Susceptibility to Cell Wall Antibiotics. Antimicrobial agents and chemotherapy. 2013 Sep; 57(9):4267-4275. doi:10.1128/AAC.00794-13. PMID:23796923
Inaoka T, Ochi K Undecaprenyl pyrophosphate involvement in susceptibility of Bacillus subtilis to rare earth elements. Journal of bacteriology. 2012 Oct; 194(20):5632-7. . PMID:22904278
Guo RT, Ko TP, Chen AP, Kuo CJ, Wang AH, Liang PH Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate, and farnesyl thiopyrophosphate: roles of the metal ion and conserved residues in catalysis. The Journal of biological chemistry. 2005 May 27; 280(21):20762-74. . PMID:15788389

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Time of last update: 2025-06-19 21:44:54

Author of last update: Jstuelk