uppS

168

undecaprenyl pyrophosphate synthetase, provides the precursor for the carrier lipid, undecaprenyl phosphate (C55-P), which is required for cell wall synthesis

Locus: BSU_16530

Molecular weight: 29.63 kDa

Isoelectric point: 5.41

Protein length: 260 aa

Gene length: 783 bp

Function: cell wall biosynthesis

Product: undecaprenyl pyrophosphate synthetase

Essential: yes

E.C.: 2.5.1.31

Synonyms: uppS, yluA

Genomic Context

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List of homologs in different organisms, belongs to COG0020 (Galperin et al., 2021)

Gene

Coordinates: 1,721,214  1,721,996

Phenotypes of a mutant

• essential PubMed
• uppS is dispensable in wall-less L-forms PubMed
• the uppS86 (C257T) mutation provides resistance to rare earth metals such as scandium and to bacitracin PubMed
• inactivation suppresses the essentiality of mbl PubMed

The protein

Catalyzed reaction/ biological activity

• catalyzes eight consecutive condensation reactions of isopentenyl pyrophosphate (IPP) with farnesyl pyrophosphate (FPP) to form undecaprenyl pyrophosphate

Protein family

• UPP synthetase family (single member, according to UniProt)

Structure

• 1X09 (PDB) (the protein from E. coli, 49% identity, 77% similarity) PubMed
• O31751 (AlphaFold)

Expression and Regulation

Operons

• Genes: uppS-cdsA-ispC-rasP-proS-polC

  Description: PubMed

Biological materials

Mutant

• MGNA-B080 (yluA::erm), available at the NBRP B. subtilis, Japan

References

Englehart K, Dworkin JBacillus subtilis MurJ and Amj Lipid II flippases are not essential for growth.Journal of bacteriology. 2025 Apr 4; :e0007825. PMID: 40183557
Koo BM, Todor H, Sun J, van Gestel J, Hawkins JS, Hearne CC, Banta AB, Huang KC, Peters JM, Gross CAComprehensive double-mutant analysis of the Bacillus subtilis envelope using double-CRISPRi.bioRxiv : the preprint server for biology. 2024 Aug 16; . PMID: 39185233
O'Reilly FJ, Graziadei A, Forbrig C, Bremenkamp R, Charles K, Lenz S, Elfmann C, Fischer L, Stülke J, Rappsilber JProtein complexes in cells by AI-assisted structural proteomics.Molecular systems biology. 2023 Feb 23; :e11544. PMID: 36815589
Guo T, Herman JKMagnesium Modulates Bacillus subtilis Cell Division Frequency.Journal of bacteriology. 2022 Dec 14; :e0037522. PMID: 36515540
Workman SD, Day J, Farha MA, El Zahed SS, Bon C, Brown ED, Organ MG, Strynadka NCJStructural Insights into the Inhibition of Undecaprenyl Pyrophosphate Synthase from Gram-Positive Bacteria.Journal of medicinal chemistry. 2021 Sep 2; . PMID: 34473495
Wang Y, Desai J, Zhang Y, Malwal SR, Shin CJ, Feng X, Sun H, Liu G, Guo RT, Oldfield E Bacterial Cell Growth Inhibitors Targeting Undecaprenyl Diphosphate Synthase and Undecaprenyl Diphosphate Phosphatase. ChemMedChem. 2016 Oct 19; 11(20):2311-2319. doi:10.1002/cmdc.201600342. PMID:27578312
Kawai Y, Mercier R, Errington J Bacterial cell morphogenesis does not require a preexisting template structure. Current biology : CB. 2014 Apr 14; 24(8):863-7. doi:10.1016/j.cub.2014.02.053. pii:S0960-9822(14)00251-6. PMID:24704074
Hess BM, Xue J, Markillie LM, Taylor RC, Wiley HS, Ahring BK, Linggi B Coregulation of Terpenoid Pathway Genes and Prediction of Isoprene Production in Bacillus subtilis Using Transcriptomics. PloS one. 2013; 8(6):e66104. doi:10.1371/journal.pone.0066104. PMID:23840410
Lee YH, Helmann JD Reducing the Level of Undecaprenyl Pyrophosphate Synthase Has Complex Effects on Susceptibility to Cell Wall Antibiotics. Antimicrobial agents and chemotherapy. 2013 Sep; 57(9):4267-4275. doi:10.1128/AAC.00794-13. PMID:23796923
Inaoka T, Ochi K Undecaprenyl pyrophosphate involvement in susceptibility of Bacillus subtilis to rare earth elements. Journal of bacteriology. 2012 Oct; 194(20):5632-7. . PMID:22904278
Guo RT, Ko TP, Chen AP, Kuo CJ, Wang AH, Liang PH Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate, and farnesyl thiopyrophosphate: roles of the metal ion and conserved residues in catalysis. The Journal of biological chemistry. 2005 May 27; 280(21):20762-74. . PMID:15788389