uppS

168

undecaprenyl pyrophosphate synthetase, provides the precursor for the carrier lipid, undecaprenyl phosphate (C55-P), which is required for cell wall synthesis

locus: BSU_16530

Molecular weight: 29.63 kDa

pI: 5.41

Protein length: 260 aa

Gene length: 783 bp

function: cell wall biosynthesis

product: undecaprenyl pyrophosphate synthetase

essential: yes

ec: 2.5.1.31

synonyms: uppS, yluA

List of homologs in different organisms, belongs to COG0020 (Galperin et al., 2021)

Gene

Coordinates: 1,721,214  1,721,996

Phenotypes of a mutant

• essential [Pubmed|22904278]
• [gene|4A637EB9B400D30E5C6246D1E0EB5E6A30F38A16|uppS] is dispensable in wall-less L-forms [Pubmed|24704074]
• the [gene|4A637EB9B400D30E5C6246D1E0EB5E6A30F38A16|uppS]86 (C257T) mutation provides resistance to rare earth metals such as scandium and to bacitracin [Pubmed|22904278]
• inactivation suppresses the essentiality of [gene|6ED386D49F973C1F6B1076974F54275DD583C9D3|mbl] [pubmed|39185233]

The protein

Catalyzed reaction/ biological activity

• catalyzes eight consecutive condensation reactions of isopentenyl pyrophosphate (IPP) with farnesyl pyrophosphate (FPP) to form undecaprenyl pyrophosphate

Protein family

• UPP synthetase family (single member, according to UniProt)

Structure

• [PDB|1X09] (the protein from ''E. coli'', 49% identity, 77% similarity) [Pubmed|15788389]
• [AF|O31751]

Expression and Regulation

Operons

• genes: [gene|4A637EB9B400D30E5C6246D1E0EB5E6A30F38A16|uppS]-[gene|7E7F6BBA03C443A5AFA05A6D9352FEF1212A5AF6|cdsA]-[gene|58CDF08A8F05C0BC25EB6E400316F85FD0F307E5|ispC]-[gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP]-[gene|848570563D3C92A8E1E6AB4DA530388D8AE94E75|proS]-[gene|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B080 (yluA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1079 NBRP B. subtilis, Japan]

References

• 22904278,23796923,15788389,23840410,24704074,27578312,34473495,36515540,36815589,39185233,40183557