mhqN

168

similar to NAD(P)H nitroreductase

Locus: BSU_05480

Molecular weight: 23.41 kDa

Isoelectric point: 5.56

Protein length: 206 aa

Gene length: 621 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: mhqN, ydfN

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0778 (Galperin et al., 2021)

Gene

Coordinates: 596,478  597,098

The protein

Protein family

• nitroreductase family (according to UniProt)

Structure

• 3BEM (PDB)
• P96692 (AlphaFold)

Expression and Regulation

Operons

• Genes: mhqN-mhqO-mhqP

  Description: PubMed

  Regulation

  • induced by catechol and 2-methylhydroquinone (MhqR) PubMed

  Regulatory mechanism

  • MhqR: repression, PubMed, in mhqR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-C153 (ydfN::erm), available at the NBRP B. subtilis, Japan
• BKE05480 (mhqN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACGCTACACTCCCTTT,  downstream forward: _UP4_TATATGTAAAAGGAGCGGAT
• BKK05480 (mhqN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTACGCTACACTCCCTTT,  downstream forward: _UP4_TATATGTAAAAGGAGCGGAT

LacZ fusion

• BP1112: Promotor-lacZ fusion, available at Jörg Stülke's and Fabian Commichau's labs

References

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