ybgG

168

homocysteine methyltransferase

Locus: BSU_02410

Molecular weight: 34.58 kDa

Isoelectric point: 4.86

Protein length: 315 aa

Gene length: 948 bp

Function: biosynthesis of methionine

Product: homocysteine methyltransferase

Essential: no

Synonyms: ybgG

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2040 (Galperin et al., 2021)

Gene

Coordinates: 261,656  262,603

The protein

Catalyzed reaction/ biological activity

• S-methyl-L-methionine + L-homocysteine --> 2 L-methionine (according to UniProt)

Domains

• Hcy-binding domain (aa 2-309) (according to UniProt)

Cofactors

• Zn²⁺ (according to UniProt)

Structure

• 5DML (PDB) (from Escherichia coli, 52% identity) PubMed
• O31463 (AlphaFold)

Expression and Regulation

Operons

• Genes: ybgG

  Description: PubMed

• Genes: ybgG-ybgF

  Description: PubMed

Biological materials

Mutant

• MGNA-B941 (ybgG::erm), available at the NBRP B. subtilis, Japan
• BKE02410 (ybgG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGAATGTACCGATGCCT,  downstream forward: _UP4_TGACTTATCGGAATAGTTTG
• BKK02410 (ybgG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGAATGTACCGATGCCT,  downstream forward: _UP4_TGACTTATCGGAATAGTTTG

References

Loading