rluB

168

ribosomal large subunit pseudouridine synthase B

Locus: BSU_23160

Molecular weight: 25.88 kDa

Isoelectric point: 10.25

Protein length: 244 aa

Gene length: 735 bp

Function: 23S rRNA modification

Product: pseudouridine synthase

Essential: no

E.C.: 5.4.99.22

Synonyms: rluB, ypuL

Genomic Context

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List of homologs in different organisms, belongs to COG1187 (Galperin et al., 2021)

Gene

Coordinates: 2,421,438  2,422,172

The protein

Catalyzed reaction/ biological activity

• uridine²⁶⁰⁵ in 23S rRNA --> pseudouridine²⁶⁰⁵ in 23S rRNA (according to E. coli RluB)

Protein family

• S4 RNA-binding domain superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
• pseudouridine synthase RsuA family (with YtzG, according to UniProt)

Domains

• S4 RNA-binding domain (aa 2-68) (according to UniProt)

Structure

• 4LAB (PDB) (from E. coli, 40% identity) PubMed
• P35159 (AlphaFold)

Expression and Regulation

Operons

• Genes: rluB-resA-resB-resC-resD-resE

  Description: PubMed

Biological materials

Mutant

• MGNA-A106 (ypuL::erm), available at the NBRP B. subtilis, Japan
• BKE23160 (rluB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCTTTCACCTCAAGTA,  downstream forward: _UP4_TAAATTTCACATAACCTTCA
• BKK23160 (rluB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCTTTCACCTCAAGTA,  downstream forward: _UP4_TAAATTTCACATAACCTTCA

References

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