skfE

168

ABC transporter (ATP-binding protein), export of the spore killing factor

Locus: BSU_01950

Molecular weight: 26.75 kDa

Isoelectric point: 6.34

Protein length: 239 aa

Gene length: 720 bp

Function: export of the spore killing factor SkfA

Product: ABC transporter (ATP-binding protein)

Essential: no

E.C.: 3.6.3.25

Synonyms: skfE, ybdA

Genomic Context

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List of homologs in different organisms, belongs to COG1131 (Galperin et al., 2021)

Gene

Coordinates: 216,913  217,632

Phenotypes of a mutant

• inactivation of ''skfE'' reduces sporulation efficiency to 54% that of wild type cells PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + sulfate --> ADP + H⁺ + phosphate + sulfate (according to UniProt)
• ATP + H₂O + thiosulfate --> ADP + H⁺ + phosphate + thiosulfate (according to UniProt)

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• ABC transporter domain (aa 4-232) (according to UniProt)

Structure

• 4RVC (PDB) (from Geobacillus kaustophilus, 33% identity) PubMed
• O31427 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: skfA-skfB-skfC-skfE-skfF-skfG-skfH

  Description: PubMed

  Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed

  Regulatory mechanism

  • Spo0A: activation, PubMed, in spo0A regulon
  • AbrB: repression, PubMed, in abrB regulon
  • PhoP: activation, PubMed, in phoP regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • Northern blotting during during phosphate limitation showed an intense 0.25 kb 'skfA'-specific transcript, and a weaker 6.5 kb 'skfA-skfB-skfC-skfE-skfF-skfG-skfH' transcript.

Biological materials

Mutant

• MGNA-C515 (ybdA::erm), available at the NBRP B. subtilis, Japan
• BKE01950 (skfE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGCATCAAATCTTCTCCTA,  downstream forward: _UP4_TAATATCATTTCTTTTTTAT
• BKK01950 (skfE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGCATCAAATCTTCTCCTA,  downstream forward: _UP4_TAATATCATTTCTTTTTTAT

References

Reviews

González-Pastor JE Cannibalism: a social behavior in sporulating Bacillus subtilis. FEMS microbiology reviews. 2011 May; 35(3):415-24. doi:10.1111/j.1574-6976.2010.00253.x. PMID:20955377

Original Publications

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