sspC

sspC
168

small acid-soluble spore protein (minor alpha/beta-type SASP)

Locus
BSU_19950
Molecular weight
7.62 kDa
Isoelectric point
9.1
Protein length
Gene length
Function
protection of spore DNA
Product
small acid-soluble spore protein (minor alpha/beta-type SASP)
Essential
no
Synonyms
sspC

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5852 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,156,239  2,156,457
The protein
Protein family
Alpha/beta-type SASP family (according to UniProt)
Structure
2Z3X (PDB) (in complex with DNA)
Paralogous protein(s)
Expression and Regulation
Operons
Genes
Description
Regulation
expressed late during sporulation in the forespore (SigG) PubMed
Sigma factors
SigG: sigma factor, PubMed, in sigG regulon
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sspC

2025-07-16 17:37:25

ghost

76

604a19bbfe57965113f8ddf9772b07212898c97a

5C68E83A498E2B21271D05450F39720723EB858F

Biological materials
Mutant
BKE19950 (sspC::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATCTTATTCATCTCCTAAA,  downstream forward: _UP4_TTAGCTCAACAAAACATGGGC
BKK19950 (sspC::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATCTTATTCATCTCCTAAA,  downstream forward: _UP4_TTAGCTCAACAAAACATGGGC
References
Reviews
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Original Publications
Lee KS, Bumbaca D, Kosman J, Setlow P, Jedrzejas MJ Structure of a protein-DNA complex essential for DNA protection in spores of Bacillus species. Proceedings of the National Academy of Sciences of the United States of America. 2008 Feb 26; 105(8):2806-11. doi:10.1073/pnas.0708244105. PMID:18287075
Steil L, Serrano M, Henriques AO, Völker U Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis. Microbiology (Reading, England). 2005 Feb; 151(Pt 2):399-420. . PMID:15699190
Frenkiel-Krispin D, Sack R, Englander J, Shimoni E, Eisenstein M, Bullitt E, Horowitz-Scherer R, Hayes CS, Setlow P, Minsky A, Wolf SG Structure of the DNA-SspC complex: implications for DNA packaging, protection, and repair in bacterial spores. Journal of bacteriology. 2004 Jun; 186(11):3525-30. . PMID:15150240
Kosman J, Setlow P Effects of carboxy-terminal modifications and pH on binding of a Bacillus subtilis small, acid-soluble spore protein to DNA. Journal of bacteriology. 2003 Oct; 185(20):6095-103. . PMID:14526021
Sohail A, Hayes CS, Divvela P, Setlow P, Bhagwat AS Protection of DNA by alpha/beta-type small, acid-soluble proteins from Bacillus subtilis spores against cytosine deamination. Biochemistry. 2002 Sep 24; 41(38):11325-30. . PMID:12234173
Hayes CS, Setlow P An alpha/beta-type, small, acid-soluble spore protein which has very high affinity for DNA prevents outgrowth of Bacillus subtilis spores. Journal of bacteriology. 2001 Apr; 183(8):2662-6. . PMID:11274127
Hayes CS, Alarcon-Hernandez E, Setlow P N-terminal amino acid residues mediate protein-protein interactions between DNA-bound alpha /beta -type small, acid-soluble spore proteins from Bacillus species. The Journal of biological chemistry. 2001 Jan 19; 276(3):2267-75. . PMID:11044450
Hayes CS, Illades-Aguiar B, Casillas-Martinez L, Setlow P In vitro and in vivo oxidation of methionine residues in small, acid-soluble spore proteins from Bacillus species. Journal of bacteriology. 1998 May; 180(10):2694-700. . PMID:9573155
Carrillo-Martinez Y, Setlow P Properties of Bacillus subtilis small, acid-soluble spore proteins with changes in the sequence recognized by their specific protease. Journal of bacteriology. 1994 Sep; 176(17):5357-63. . PMID:8071212
Connors MJ, Setlow P Cloning of a small, acid-soluble spore protein gene from Bacillus subtilis and determination of its complete nucleotide sequence. Journal of bacteriology. 1985 Jan; 161(1):333-9. . PMID:2981806
Nicholson WL, Sun DX, Setlow B, Setlow P Promoter specificity of sigma G-containing RNA polymerase from sporulating cells of Bacillus subtilis: identification of a group of forespore-specific promoters. Journal of bacteriology. 1989 May; 171(5):2708-18. . PMID:2468649

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Time of last update: 2025-07-28 09:39:50

Author of last update: Jstuelk