int

168

integrase

Locus: BSU_04800

Molecular weight: 43.32 kDa

Isoelectric point: 9.76

Protein length: 368 aa

Gene length: 1107 bp

Function: excision of the conjugative transposon ICEBs1 from the trnS-leu2 locus

Product: integrase

Essential: no

Synonyms: int, ydcL

Genomic Context

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Categories containing this gene/protein

Prophages and mobile genetic elements, Mobile genetic elements, ICEBs1

List of homologs in different organisms, belongs to COG4974 (Galperin et al., 2021)

Gene

Coordinates: 529,505  530,611

The protein

Protein family

phage integrase family (according to UniProt)

Structure

4A8E (PDB) (from Pyrococcus abyssi, 25% identity) PubMed
P96629 (AlphaFold)

Expression and Regulation

Operons

Genes: immR-immA-int

Description: PubMed

Biological materials

Mutant

BKE04800 (int::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTATTTCTCCTTTAAC,  downstream forward: _UP4_TAGGTAAGTGGGCAGTTTGT
BKK04800 (int::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTATTTCTCCTTTAAC,  downstream forward: _UP4_TAGGTAAGTGGGCAGTTTGT

References

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