moeB

168

molybdopterin biosynthesis protein

Locus: BSU_14270

Molecular weight: 37.36 kDa

Isoelectric point: 6.09

Protein length: 339 aa

Gene length: 1020 bp

Function: nitrate respiration

Product: unknown

Essential: no

E.C.: null

Synonyms: moeB

Genomic Context

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List of homologs in different organisms, belongs to COG0476 (Galperin et al., 2021)

Gene

Coordinates: 1,496,155 → 1,497,174

The protein

Catalyzed reaction/ biological activity

• [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H⁺ --> [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate (according to UniProt)

Protein family

• HesA/MoeB/ThiF family (with ThiF and YrvM, according to UniProt)

Structure

• 1ZFN (PDB) (ThiF from E. coli, 35% identity, 48% similarity) PubMed
• O31702 (AlphaFold)

Paralogous protein(s)

• ThiF

Expression and Regulation

Operons

• Genes: mobA-moeB-moeA-mobB-moaE-moaD

  Description: PubMed

Biological materials

Mutant

• BKE14270 (ΔmoeB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAAACCTGTTCTCCTTA,  downstream forward: _UP4_CTGTAACAACAGGAGGGGTC
• BKK14270 (ΔmoeB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAAACCTGTTCTCCTTA,  downstream forward: _UP4_CTGTAACAACAGGAGGGGTC

References

Reviews

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Original Publications

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