Difference between revisions of "QueD"
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|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification | |style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13730 queD] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 5.944 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 5.944 | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[queC]]'', ''[[queE]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[queC]]'', ''[[queE]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13730 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13730 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13730 DNA_with_flanks] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:ykvK_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:ykvK_context.gif]] | ||
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | ||
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| − | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=queD_1440100_1440549_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:queD_expression.png|500px]] | + | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=queD_1440100_1440549_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:queD_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13730]] |
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|} | |} | ||
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13730&redirect=T BSU13730] | ||
* '''DBTBS entry:''' no entry | * '''DBTBS entry:''' no entry | ||
| Line 93: | Line 94: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13730&redirect=T BSU13730] | ||
* '''Structure:''' | * '''Structure:''' | ||
Latest revision as of 13:33, 2 April 2014
- Description: 6-pyruvoyltetrahydropterin synthase, synthesis of the modified ribonucleotide queuosine
| Gene name | queD |
| Synonyms | ykvK |
| Essential | no |
| Product | 6-pyruvoyltetrahydropterin synthase |
| Function | tRNA modification |
| Gene expression levels in SubtiExpress: queD | |
| MW, pI | 16 kDa, 5.944 |
| Gene length, protein length | 447 bp, 149 aa |
| Immediate neighbours | queC, queE |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13730
Phenotypes of a mutant
Database entries
- BsubCyc: BSU13730
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU13730
- Structure:
- UniProt: O31676
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- repressed in the presence of queuosine (preQ1 riboswitch) PubMed
- Regulatory mechanism:
- preQ1 riboswitch: transcriptional antitermination in the absence of queuosine PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Mijeong Kang, Robert Peterson, Juli Feigon
Structural Insights into riboswitch control of the biosynthesis of queuosine, a modified nucleotide found in the anticodon of tRNA.
Mol Cell: 2009, 33(6);784-90
[PubMed:19285444]
[WorldCat.org]
[DOI]
(I p)
Nenad Cicmil, Lu Shi
Crystallization and preliminary X-ray characterization of queD from Bacillus subtilis, an enzyme involved in queuosine biosynthesis.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2008, 64(Pt 2);119-22
[PubMed:18259064]
[WorldCat.org]
[DOI]
(I p)
Adam Roth, Wade C Winkler, Elizabeth E Regulski, Bobby W K Lee, Jinsoo Lim, Inbal Jona, Jeffrey E Barrick, Ankita Ritwik, Jane N Kim, Rüdiger Welz, Dirk Iwata-Reuyl, Ronald R Breaker
A riboswitch selective for the queuosine precursor preQ1 contains an unusually small aptamer domain.
Nat Struct Mol Biol: 2007, 14(4);308-17
[PubMed:17384645]
[WorldCat.org]
[DOI]
(P p)
Matthew R Schaab, Brett M Barney, Wilson A Francisco
Kinetic and spectroscopic studies on the quercetin 2,3-dioxygenase from Bacillus subtilis.
Biochemistry: 2006, 45(3);1009-16
[PubMed:16411777]
[WorldCat.org]
[DOI]
(P p)
John S Reader, David Metzgar, Paul Schimmel, Valérie de Crécy-Lagard
Identification of four genes necessary for biosynthesis of the modified nucleoside queuosine.
J Biol Chem: 2004, 279(8);6280-5
[PubMed:14660578]
[WorldCat.org]
[DOI]
(P p)
